Journal: STAR Protocols

Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

doi: 10.1016/j.xpro.2026.104471

Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

Techniques: Flow Cytometry, Isolation, Staining

Journal: STAR Protocols

Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach

doi: 10.1016/j.xpro.2026.104439

Figure Lengend Snippet: 96-well plate setup for K D probe determination

Article Snippet: 96-well conical black plate , Thermo Fisher ScientificTM , #249945.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach

doi: 10.1016/j.xpro.2026.104439

Figure Lengend Snippet: 96-well plate setup for FASST assay

Article Snippet: 96-well conical black plate , Thermo Fisher ScientificTM , #249945.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach

doi: 10.1016/j.xpro.2026.104439

Figure Lengend Snippet:

Article Snippet: 96-well conical black plate , Thermo Fisher ScientificTM , #249945.

Techniques: Recombinant, Software, Spectrophotometry, Fluorescence, Membrane, Sterility